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Thermo Scientific™ ProPac™ SAX-10 Quaternary Ammonium Ion Exchange HPLC Column, 10 μm, 22 mm x 250 mm
Column Format: Semi-Preparative Column; Pore Size: Nonporous; Column Phase: Ion Exchange
Brand: Thermo Scientific™ SP5594
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Description
Use Thermo Scientific™ ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants. Based on a nonporous 10µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.
Maximizing Resolution
- ProPac SAX-10 LC column is a strong anion-exchanger with a quaternary amine functional group.
- Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.
Superior Performance for Protein Analysis
- Ideal for characterization and quality control assessment of closely-related protein variants
- Unequaled high-resolution separations
- High-efficiency peaks with excellent recovery
- Higher capacity than other columns based on nonporous particles
- Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing
Specifications
Semi-Preparative Column | |
1 mL/min | |
3000 psi (200 bar) | |
10 μm | |
Nonporous | |
Quaternary Ammonium | |
Spherical, Nonporous Polymer | |
Ion Exchange |
22 mm | |
250 mm | |
pH 2 to 12, 80% Compatible with Acetonitrile, Acetone, Methanol, Compatible with Nonionic and Zwitterionic Detergents, Incompatible with Anionic Detergents | |
2 to 12 | |
60°C | |
ProPac | |
Ion Exchange |