Thermo Scientific™ ProPac™ SAX-10 Quaternary Ammonium Ion Exchange HPLC Column, 10 μm, 22 mm x 250 mm

Column Format: Semi-Preparative Column; Pore Size: Nonporous; Column Phase: Ion Exchange

Brand:  Thermo Scientific™ SP5594

Product Code. 11392483

Description

Use Thermo Scientific™ ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants. Based on a nonporous 10µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Maximizing Resolution

• ProPac SAX-10 LC column is a strong anion-exchanger with a quaternary amine functional group.
• Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.

Superior Performance for Protein Analysis

• Ideal for characterization and quality control assessment of closely-related protein variants
• Unequaled high-resolution separations
• High-efficiency peaks with excellent recovery
• Higher capacity than other columns based on nonporous particles
• Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing

Specifications

• Column Type: Ion Exchange
• Phase: Ion Exchange
• Diameter (Metric): 22 mm
• Length (Metric): 250 mm
• Mobile Phase Compatibility: pH 2 to 12, 80% Compatible with Acetonitrile, Acetone, Methanol, Compatible with Nonionic and Zwitterionic Detergents, Incompatible with Anionic Detergents
• pH: 2 to 12
• Temperature: 60°C
• Product Line: ProPac

• Packing Material: Spherical, Nonporous Polymer
• Column Format: Semi-Preparative Column
• Flow Rate: 1 mL/min
• Max. Pressure: 3000 psi (200 bar)
• Particle Size: 10 μm
• Pore Size: Nonporous
• Stationary Phase: Quaternary Ammonium