Progesterone Receptor, clone: KMC912, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9764-80

Product Code. 15527656

Description

The progesterone receptor is a member of the Steroid Hormone Receptor Superfamily. It exists in two distinct isoforms in human cells. PR-B is the transcriptionally active form and is responsible for activating genes for the maintainence of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. Phospholabeling studies have shown that PR is phosphorylated in at least 9 different locations in response to progestins, which suggests that PR affects many different cellular pathways.

Description: This KMC912 monoclonal antibody reacts with human progesterone receptor (PgR, PR), a member of a superfamily of nuclear receptors that are ligand-dependent transcriptional regulators. The human PgR exists in alpha and beta forms, 94kDa and 120kDa respectively. In most human cells, the alpha and beta forms are expressed at similar levels and predominately form heterodimers. Progestin binding to PgR causes a conformational change, allowing dissociation of bound chaperone proteins and subsequent dimerization with either PgRa or PgRb. Following activation, dimerized PgR can directly bind to DNA through progestin response elements (PRE) leading to chromatin remodeling and subsequent downregulation or transcription of the target gene.The PgR plays a key role in controlling gene expression in breast, uterine, brain, and cardiovascular tissue during development. The presence of the PgR in breast tissue is indicative of improved survival and a better response to endocrine therapy. In breast and endometrial cancer progression, a predominance of either the alpha or beta form occurs, suggesting disregulation in the PgRa:PgRb ratio is an early event in cancer. In cases of ductal carcinoma in situ and invasive ductal carcinoma, there is predominance of the alpha form while in uterine cancer a loss of either form is common.Applications Reported: This KMC912 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.Applications Tested: This KMC912 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high or low pH antigen retrieval and can be used at less than or equal to 2.5 μg/mL. This KMC912 antibody has been tested by immunocytochemistry of methanol-fixed or formaldehyde-fixed and permeabilized human cells and can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Progesterone Receptor
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P06401
• Gene Symbols: PGR
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5241
• Content And Storage: Store at 2-8°C.
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: KMC912
• Conjugate: Unconjugated
• Gene: PGR
• Gene Alias: PR, NR3C3, PGR
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.