Applied Biosystems™ ProFlex™ Sample Blocks

Product Code.	Block Configurations	unitSize
15107105	3 x 32-Well, 2-Zone VeriFlex Blocks	Each
15117105	96-well, 6-Zone VeriFlex Block	Each
15238656	2 x 96-well Isothermal Blocks	Each
15334853	2 x 384-well Isothermal Blocks	Each
15372476	2 x Flat Blocks	Each

Product Code. 15107105 Supplier Applied Biosystems™ Supplier No. 4483638

Description

Requires

ProFlex Base Module for operation

The ProFlex PCR System is unique in its design, with interchangable blocks that allow for future changes in research requirements.

The ProFlex PCR System is unique in its design, with interchangable blocks that allow for future changes in research requirements. In a step that typically takes less than 15 seconds, the block is changed without the need for special tools or kits. ProFlex interchangable blocks are intended for use with the ProFlex PCR System and require a ProFlex Base Module for operation.

• The ProFlex 96-well Sample Block has one 96-well, 0.2-mL module
• The ProFlex 2 x 96-well Sample Block has two 96-well, 0.2-mL modules
• The ProFlex 2 x 384-well Sample Block has two 384-well, 0.02-mL modules
• The ProFlex 2 x Flat Sample Block has two flat modules and is optimized for use with TaqMan OpenArray Genotyping plates
• The ProFlex 3 x 32-Well Sample Block has three independently-controlled, 32-well, 0.2-mL modules, allowing you to share your instrument with two other people or run three experiments at once

Specifications

• Thermal Accuracy: ±0.25°C (35°C to 99.9°C)
• Content And Storage: Store at room temperature.
  
  
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): ProFlex PCR System
• Thermal Uniformity: <0.5°C (20 sec. after reaching 95°C)
• Dimensions: 22 x 13 x 10.6 in. (56.5 x 33 x 27.2 cm)
• Capacity: 3 x 32-well, 0.2 mL
• Voltage: 100/240 V
• Product Type: Thermal Cycler
• Thermal Range: 0°C to 100°C
• User Interface: USB, WiFi, Cloud, Ethernet
• Max. Heating Rate (Thermal Block): 6°C/sec. (Block), 4.4°C/sec. (Sample)
• Quantity: 1 unit
• Weight: 41 lb. (18.8 kg)
• Block Configurations: 3 x 32-Well, 2-Zone VeriFlex Blocks

Frequently Asked Questions (FAQs)

How do I fix issues with screen freezing on the ProFlex PCR System?

Make sure you have the most up-to-date firmware version, as this has fixed many screen-freezing issues. The latest firmware can be downloaded from here (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/proflex-pcr-system.html). If you cannot get access to the main menu, try turning off the machine, letting it sit for 1 minute, and restarting it without any block attached.

How do I get the ProFlex PCR System to connect to the Cloud and mobile app?

Please follow these directions on how to set up Cloud and mobile connectivity in the ProFlex PCR System:

1. Ensure that the instrument is Cloud-enabled

Any ProFlex PCR System can be connected to Thermo Fisher Connect as long as it is using firmware version 1.2 or higher. You can check your current firmware version by:

a) Going to the Settings menu.
b) Selecting “About Instrument”.

2. Create a Thermo Fisher Connect account

a) From your Internet browser, go to thermofisher.com/connect
b) If you already have an account with Thermo Fisher Scientific, log in using the same name and password.
c) If you do not already have an account, follow the prompts to create one.
d) Once you have logged in to thermofisher.com/connect, an email will automatically be sent to you to verify your email address.
e) Open your email and click on the link to visit the Connect page to verify your account.
f) If you have done this successfully, you will receive a confirmation email telling you that you have connected to Thermo Fisher Connect.

3. Connect your ProFlex PCR machine to the internet using a wireless or wired connection.
-Note: Please refer to Chapter 2 of the ProFlex PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007697.pdf) for instructions on setting up of a wireless or wired connection.

4. Connect the instrument to Thermo Fisher Cloud

a) Touch Sign In.
b) Select Administrator when asked to choose screen name.
c) Log in. (The default password for the Administrator account is “password”.)
d) You will then be redirected back to the home screen. Go to Settings.
e) Select Remote Service.
f) Turn “on” Remote Service.
g) Touch My Profile on the home screen.
h) Touch the Cloud tab and enter your Thermo Fisher Connect account login and password.
i) Select Link Account. Upon successful authentication, the Administrator is linked to Thermo Fisher Connect.

-Subsequently, any user can log in and link the instrument to their Thermo Fisher Connect account by following steps 3-9 above.
-Note: The administrator account must be activated first to connect the instrument to the Cloud. Afterward, multiple users can create unique user profiles using their own login information. Each unique profile must have a unique Thermo Fisher Connect account.

5. Set mobile connectivity in a mobile device

a) Download and install the “Instrument Connect Mobile Application” on your mobile device.

-For an iPad™ or iPhone™ mobile device, download the application from the App Store™ by searching for ““Instrument Connect”.
-For Android™ devices, download the application from Google™ Play by searching for “Instrument Connect”.

b) Log in to the Instrument Connect mobile application using your Thermo Fisher Connect login and password.

-Once logged in, any Cloud-enabled instrument that has been linked to that account can be monitored.

Further information can be found here: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/remote_connectivity_with_InstrumentConnect_PI.pdf

How do I get the wireless connection for my ProFlex PCR System?

First, make sure you are running the most up to date firmware. Newer firmware versions have fixed many wireless and mobile app issues. Firmware updates can be found here: https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/proflex-pcr-system.html.

To set up the wireless connection in the ProFlex PCR System, please follow the steps outlined below:
1.Power on the ProFlex Instrument, and in the Home screen, touch Settings.
2.In the Settings screen, touch Device Settings.
3.In the Device Settings screen, touch Network Connection. In the Network Connection Type screen, touch Wireless. The Search Wireless Network screen will come up and the wireless symbol will appear active. Touch Cancel to exit the screen. Note: During initial setup, if you selected the Wired option in the Network Connection Type screen, you will be required to enter your IP address if you selected the Static IP wired option. If you selected the Dynamic IP wired option, the IP address is automatically populated.
4.Once a wireless connection has been detected, a list of the available networks will be displayed in the Choose Network screen. Touch the network name of your choice or touch Join Other Network.
5.In the Find and Join a Network screen, enter the name and security type of the network. Note: When you touch in the Network Name field, a keypad will come up to facilitate making the entry.
6.From the Security type drop-down menu, touch to select the security type and enter the relevant information in the screen.
Note: Contact your IT Systems Administrator for information on security type.
You can select from the following options:
-WEP
-WPA Personal
-WPA2 Personal
-WPA Enterprise
-WPA2 Enterprise
7.Touch Join to continue or Cancel to exit from the Find and Join a Network screen.
8.Depending on the security type you have selected, enter the appropriate passwords and touch Join.
If you entered the correct information, the Network Connection Complete screen will appear. Touch OK to continue. If you entered incorrect information, the Network Connection Failed screen will come up and you will be required to go back to security type screen to resolve the issue. Touch OK to continue.

My tubes are melting. What is wrong?

This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).

Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)

How do I program an infinite hold in the ProFlex PCR System?

To add an infinite hold, select the infinity symbol for program.

How do the VeriFlex Blocks work on the ProFlex PCR System?

In the ProFlex PCR System, the 96-well and 3 x 32-well blocks feature innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks (2 on each 32-well block for 3 x 32-well) provide maximal versatility and flexibility and offer two key benefits:

1.PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2.Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

What is the Auto Delta feature on the ProFlex PCR System?

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.

What is the ramp rate of the ProFlex PCR System?

The 96-well and 3 x 32-well system have a max block ramp rate of 6.0 degrees C/sec and the dual 96-well and dual flat blocks have a max block ramp rate of 2.0 degrees C/sec.

What types of plastics can I use with the ProFlex PCR System?

The plates and tubes will be specific to the block type. Please refer to our PCR Tubes, Plates & Accessories Plastics Selection Guide (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.

What types of blocks are available on the ProFlex PCR System?

The ProFlex PCR System has 4 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well independent block. This block allows up to 3 experiments to be run simultaneously, completely independently of each other and without interference. A dual 96-well block is available for your high throughput sequencing needs and a dual flat block is available to support OpenArray plate technology for genotyping analysis on the QuantStudio 12K Flex Real-Time PCR System.

How are the VeriFlex zones arranged on the ProFlex thermal cycler?

On the ProFlex thermal cycler, there are two formats available with VeriFlex blocks. The 96-well, 0.2 mL VeriFlex block has 6 temperature zones. The 3 x 32 well VeriFlex bock has 2 temperature zones. In both formats, the temperature zones are orientated by vertical columns (i.e., on the 3 x 32 well block, columns 1 and 2 are one temperature zone; columns 3 and 4 are the next temperature zone).