Invitrogen™ Human proBNP/NPPB ELISA Kit

Product Code. 17708013

Brand: Invitrogen™ EHPRONPPBX10

Description

ELISA

In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aasignal peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Specifications

• Accession Number: P16860
• Assay Range: 0.137 to 100 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4879
• Gene Symbol: NPPB
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: pro-Brain Natriuretic Peptide
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins