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PRKCH (PKC eta) Recombinant Human Protein

PRKCH (PKC eta)

Brand:  Invitrogen P2633

Code : 50

Additional Details : Weight : 3.18000kg

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Product Code. 10073392

  • £250.00 / Each

Estimated Shipment
11-12-2020

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The PKC family of serine⁄threonine kinases, including PRKCH (PKC eta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_006246
Kinase and inhibitors
Kinase assay
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
82.2kDa
92% by SDS-PAGE
–80°C
1 Each
PRKCH (PKC eta) Recombinant Human Protein
PKC
610nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2μg/mL
His Tag
Baculovirus
Serine/threonine kinase
0.33mg/mL
23683, 5583
PRKCH (PKC eta) Recombinant Human Protein
5μg
For research use only. Not for use in diagnostic procedures
PRKCH (PKC eta)
PRKCH (PKC eta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
PRKCH (PKC eta) Recombinant Human Protein
Human
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For Research Use Only. Not for use in diagnostic procedures.