Eurofins DiscoverX PRK2 Protein, Active

Product Code. 17380324

Brand: Eurofins DiscoverX 14549

Description

Active, Recombinant human PRK2, residues 501-end, containing N-terminal 6His tag, for use in Kinase Assays

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis.

GenBank NM_006256

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 1 mM benzamidine, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03% Brij-35.
• Molecular Weight (g/mol): 59.9 kDa
• Quantity: 10 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal 6His
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥51%
• Protein: PRK2, active