Invitrogen™ Predictor™ hERG Fluorescence Polarization Assay Kit

Product Code. 10532304

Brand: Invitrogen™ PV5365

Description

Predictor™ hERG Fluorescence Polarization Assay Kit

The Predictor™ hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the Predictor™ assay demonstrate a high correlation with those obtained from patch clamp techniques.

Specifications

• Color: Red
• Conjugate: Predictor hERG Tracer Red
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Quantity: 1 Kit
• Packaging: 384-well Plate
• Content And Storage:

  PV5365 Predictor™ hERG Fluorescence Polarization Assay Kit
  (Other materials are required separately; please refer to the protocol)
  Includes:
  • Predictor™ hERG Membranes
  • Predictor™ hERG Tracer Red
  • Predictor™ hERG Buffers
  • E-4031
  • Predictor™ hERG Fluorescence Polarization Assay Protocol
  • Certificate of Analysis
  Store the Predictor™ hERG Membranes at -80°C. The Predictor™ Tracer Red, Predictor™buffers, and E-4301 should be stored at -20°C.

• Assay Entry: Biochemical binding
• Druggable Target: Ion Channels
• Gene Symbol: hERG
• Readout: End Point
• Shipping Condition: Dry Ice
• System Type: Predictor™ System
• Target Entry: hERG
• Product Line: Predictor™
• Protein Family: Ion Channels
• Product Type: Assay Kit

For Research Use Only. Not for use in diagnostic procedures.