Thermo Scientific™ Precise™ 8%, Tris-HEPES, Protein Gel, 12-well

Separation Range: 45 to 205 kd

Brand:  Thermo Scientific™ 25220

Product Code. 11891385

Description

Thermo Scientific™ Precise Tris-HEPES Gels are precast polyacrylamide gels for protein electrophoresis (PAGE) that use Tris-HEPES running buffer, have long shelf lives, and provide compatibility with different commercial gel tanks.

Precise Gels are cast in a durable plastic cassette using a neutral-pH buffer that prevents polyacrylamide breakdown and results in a long shelf life. High-resolution staining and transfer of proteins is accomplished quickly on these 1mm thick mini gels. Gels are individually packaged in an easy-to-open plastic pouch and are ready to run with no comb or tape to remove. Following electrophoresis, the cassettes are easy to dismantle (no special tools required) without damaging the gel. Precise Gels are available in gradient (4 to 20% and 8 to 16%) and fixed (8%, 10% and 12%) concentrations and in 10-well, 12-well and 15-well formats.

Highlights:

• High-performance – unique running buffer produces excellent separation and high-resolution protein bands that stain easily using coomassie and silver stains

• Long shelf life – guaranteed 12-month stability ensures consistent performance for the duration of each package of gels

• Fast – 45-minute run-time provides results quickly; transfers efficiently to nitrocellulose and PVDF membranes in just 90 minutes

• Large-volume wells – innovative well design accommodates twice the sample volume of typical mini-gel cassette wells (10-well=50μL, 12-well=30μL; 15-well=25μL)

• Compatible – cassettes fit many popular mini-gel tanks, and gels can be loaded using standard Laemmli sample loading buffers

• Several convenient styles – choose from five gel densities and three well formats

Additional Features:

• Cassette dimensions: 10cm × 8.5cm × 4.5mm
• Gel dimensions: 8cm × 5.8cm × 1mm
• Storage conditions: 1 year at 4°C
• Stacking gel: 4% polyacrylamide
• Buffer system in gel: Tris-HCl, pH 7
• SDS content: none
• Required running buffer: Tris-HEPES-SDS (Part No. 28398)
• Recommended sample buffer: Tris-HCl-SDS (any type)

Precise Protein Gels are compatible with the following gel tanks: BioRad™ Mini-PROTEAN™ II and 3; Life Technologies XCell I, II™ and Surelock™; IBI Universal Protein System; EC 4-Cell; Hoefer Tall Mighty Small™ (SE 280); Hoefer Mighty Small™ II (SE 260/ SE 250); Hoefer Mini VE System (SE 300); C.B.S. Scientific MGV 302/402; Daiichi Mini 2-Gel and 6-Gel; GradiGel Mini 4-Cell; Fisher Biotech Vertical Minigel System.

Specifications

• Description: 8% Tris-HEPES Gels, 10 x 8.5cm, 12-Well
• GelThickness: 4.5 mm
• Product Type: Homogeneous Page
• Separation Range: 45 to 205 kDa
• Formulation: Precast 8% polyacrylamide gels for SDS-PAGE
• Sufficient For: 12 samples per gel, 30uL capacity per well
• Length (Metric): 100 mm

• Gel Percentage: 8%
• Gel Type: Tris-HEPES
• Separation of: Protein
• Wells: 12-well
• Quantity: 10/Pk.
• Well Design: Standard
• Width (Metric): 85 mm