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Invitrogen™ Power Blotter 1-Step™ Transfer Buffer (5X)
Description
Invitrogen Power Blotter 1-Step Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter System. Transfer is compatible with commonly used detection methods such as staining, chemiluminescence, or fluorescence.
Features of Power Blotter 1-Step Transfer Buffer:
• Fast—high ionic strength formulation allows for 5- to 12-minute protein transfer when used with compatible fast semi-dry blotting systems
• Optimized—designed for seamless and reliable performance with the Power Blotter System
• Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply
• Economical—no need for special or costly consumables that are required by other fast semi-dry transfer devices
When used with the Power Blotter System, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 12 minutes with efficiency that is similar to conventional western blotting techniques. Fast blotting methods require a high-current power supply, such as the Power Blotter System, and an optimized high ionic strength transfer buffer, such as Power Blotter 1-Step Transfer Buffer.
By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (∼22–23 mA/cm2) and voltage is limited to 25 V.
Power Blotter 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.
Related products
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size (Cat. No. PB3310)
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini (PB3210)
Power Blotter Pre-cut Membranes and Filters, PVDF, regular size (PB5310)
Power Blotter Pre-cut Membranes and Filters, PVDF, mini (PB4210)
Specifications
Specifications
| For Use With (Application) | Western Blot |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Mode of Transfer | Semi-Dry |
| Product Line | 1-Step |
| Type | Transfer Buffer |
| Quantity | 1 L |
| Shipping Condition | Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.
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