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Porcine IFN gamma ELISA Kits, Thermo Scientific™

Microplate kit for sandwich ELISA of porcine interferon gamma (IFN-g)

Brand:  Invitrogen EPIFNG5

Code : NEW

Additional Details : Weight : 0.01000kg

Product Code. 15398214

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
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Includes: Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description

Description

This porcine IFN-gamma ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant porcine interferon gamma (IFNγ)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 8 to 500pg/mL
  • Sensitivity: < 2pg/mL
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size:50μL
  • Specificity: No significant cross-reaction with with porcine IL-1β, IL-6, IL-8, IL-12 (p40), IL-12 (p70), or human, mouse or rat IFNγ

Specifications

Specifications

Porcine
Anti-Porcine IFNγ Pre-coated 96-well Strip Plate, 5 each
Lyophilized Recombinant Porcine IFNγ Standard, 10 vials
Standard Diluent, contains 0.1% NaN3, 5 x 12mL
Biotinylated Antibody Reagent,contains 0.1% NaN3, 5 x 12mL
30X Wash Buffer, 5 x 50mL
Streptavidin-HRP Concentrate, 5 x 75μL
Streptavidin-HRP Dilution Buffer, 5 x 14mL
TMB Substrate, 5 x 13mL
Stop Solution, contains 0.16M sulfuric acid, 5 x 13mL
Adhesive plate covers, 30 each
< 2 pg/mL
50μL
5 x 96 microplate wells
Natural and recombinant porcine interferon gamma (IFNγ)
Complete ELISA kit with coated 96-well plate
5 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with with porcine IL-1β, IL-6, IL-8, IL-12 (p40), IL-12 (p70), or human, mouse or rat IFNγ
3 hours
8 to 500pg/mL
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