Invitrogen™ POLQ Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5115130

Product Code. 17257893

Description

Antibody detects endogenous levels of total DNA Polymerase theta.

DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt].

Specifications

• Antigen: POLQ
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLQ
• Gene Alias: A430110D14Rik; Chaos1; Chromosome aberrations occurring spontaneously protein 1; DNA polymerase eta; DNA polymerase theta; DNA polymerase theta; LOW QUALITY PROTEIN: DNA polymerase theta; EC 2.7.7.7; POLH; Polq; polymerase (DNA directed), theta; polymerase (DNA) theta; PRO0327
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10721, 288079, 77782
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide
• Gene Accession No.: O75417, Q8CGS6
• Gene Symbols: POLQ
• Immunogen: A synthesized peptide derived from human POLQ(Accession O75417), corresponding to amino acid residues K1581-W1631.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG