Invitrogen™ Pokemon (LRF) Monoclonal Antibody (13E9), eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Invitrogen™ 14-3309-37

Product Code. 17100632

Description

Description: This monoclonal antibody reacts with human Leukemia/lymphoma-related factor (LRF). This 65-kDa protein is also known as POKEMON, FBI-1, and Zbtb7a. LRF is a member of the POK family of transcriptional repressors, and is expressed in hematopoietic stem cells, T and B lymphocytes, macrophages, and erythroid cells. This protein is also highly expressed in human non-Hodgkin's lymphoma tissues and diffuse large B-cell lymphomas, demonstrating its prominent role in oncogenic transformation. Studies have demonstrated that LRF is also involved in B cell lineage commitment and development by opposing Notch signaling. Moreover, LRF is critical for erythroid terminal differentiation in the bone marrow by inhibiting Bim-mediated apoptosis. Finally, LRF interacts with Bcl-6 in germinal center B cells. Applications Reported: This 13E9 antibody has been reported for use in immunoblotting (WB) and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 13E9 antibody has been tested by western blot analysis of the A20 cell line and by immunohistochemical staining of formalin-fixed paraffin embedded human tonsil tissue. This can be used at 1-10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.

Specifications

• Antigen: Pokemon (LRF)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O95365
• Gene Symbols: ZBTB7A
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51341
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 13E9
• Conjugate: Unconjugated
• Gene: ZBTB7A
• Gene Alias: 9030619K07Rik; 9130006G12Rik; AI452336; cLRF; DKFZp547O146; factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI1; FBI-1; HIV-1 1st-binding protein 1; HIV-1 inducer of short transcripts binding protein; leukemia/lymohoma related factor cLRF; leukemia/lymphoma related factor; leukemia/lymphoma related factor cLRF; leukemia/lymphoma-related factor; LRF; lymphoma related factor; MGC99631; Oczf; Osteoclast-derived zinc finger protein; POK erythroid myeloid ontogenic factor; Pokemon; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF-I-interacting peptide 21; Zbtb7; ZBTB7A; zi; zinc finger and BTB domain containing 7a; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; zinc finger and BTB domain-containing protein 7A; Zinc finger protein 857A; ZNF857A
• Host Species: Armenian Hamster
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG