Invitrogen™ POFUT1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA597014

Product Code. 16519851

Description

Immunogen sequence: SWDPAGYLLY CPCMGRFGNQ ADHFLGSLAF AKLLNRTLAV PPWIEYQHHK PPFTNLHVSY QKYFKLEPLQ AYHRVISLED FMEKLAPTHW PPEKRVAYCF EVAAQRSPDK KTCPMKEGNP FGPFWDQFHV SFNKSELFTG ISFSASYREQ WSQRRENHSC VTLLFPR; Positive Samples: HepG2, HL-60, Mouse heart, Mouse liver; Cellular Location: Endoplasmic reticulum.

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.

Specifications

• Antigen: POFUT1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POFUT1
• Gene Alias: DDD2; FUT12; GDP-fucose protein O-fucosyltransferase 1; KIAA0180; mKIAA0180; o-fucosyltransferase protein; O-Fuc-T; OFUCT1; O-FucT-1; O-FucT-1a; O-FucT-1b; O-FUT; peptide-O-fucosyltransferase 1; Pofut1; pofut1 {ECO:0000312; protein O-fucosyltransferase 1; protein O-fucosyltransferase 1 isoform 1 precursor; protein O-fucosyltransferase 1a; protein O-fucosyltransferase 1b; protein-O-fucosyltransferase 1; RGD:1303001}; zgc:110472
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 140484, 23509, 311551
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.71 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q6EV70, Q91ZW2, Q9H488
• Gene Symbols: POFUT1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_7584361).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG