missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. ELISA Substrates and Reagents
  5. Thermo Scientific™ PNPP Substrates

Thermo Scientific™ PNPP Substrates

Product Code. 11889260 Shop All Thermo Scientific Products
Click to view available options
Format:
Kit
Liqiuid
Powder
Tablet
Unit Size:
1 set
100mL
105 tablets
25g
This item is not returnable. View return policy

Product Code. 11889260

Brand: Thermo Scientific™ 34045

Please to purchase this item. Need a web account? Register with us today!

Edge
172.55 GBP valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy

Description

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

TRUSTED_SUSTAINABILITY

Specifications

Description PNPP Powder
Product Type ELISA Substrate
Quantity 25 g
Detection Method Colorimetric
Form Powder
Format Powder
Substrate Properties Chemical Substrate
Substrate Type Alkaline Phosphatase
Content And Storage Upon receipt store product at 4°C.
Shipping Condition Ambient
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.