PNK Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA528853

Product Code. 13402977

Description

Recommended positive controls: A549, HeLa, HepG2. Predicted reactivity: Mouse (82%), Rat (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Anti-human Polynucleotide Kinase Mouse Monoclonal Antibody recognizes polynucleotide kinase (PNK), a 57 kDa nuclear enzyme implicated in DNA strand repair processes that occur during DNA replication and recombination, or in response to DNA damaging agents. PNK targets the DNA 5'-hydroxy and 3'-phosphate termini for phosphorylation and dephosphorylation events. During strand breakage in human cells, normal PNK associates with other nuclear proteins to prepare the DNA termini for the enzymatic actions of DNA polymerase-beta and DNA ligase III. PNK is a key functional component in maintaining the genetic stability of human cells.

Specifications

• Antigen: PNK
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Pnkp
• Gene Alias: 1810009G08Rik; 2'(3')-polynucleotidase; AOA4; bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); MCSZ; phosphatase/kinase; PKNP; PNK; PNKP; Polynucleotide 3'-phosphatase; Polynucleotide 5'-hydroxyl-kinase; polynucleotide kinase 3'- phosphatase; polynucleotide kinase 3'-phosphatase; polynucleotide kinase-3'-phosphatase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11284
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q96T60
• Gene Symbols: Pnkp
• Immunogen: Recombinant fragment corresponding to a region within amino acids 78 and 521 of Human PNK
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG