Invitrogen™ PLZF Monoclonal Antibody (9E12), APC, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
16360734	Invitrogen™ 17-9322-82	100 μg

Description

This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintece of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor. This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7. This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 0.

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Specifications

• Antigen: PLZF
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q05516
• Gene Symbols: ZBTB16
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: ZBTB16

• Clone: 9E12
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Armenian Hamster
• Target Species: Human, Mouse
• Gene ID (Entrez): 235320, 7704
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.