Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Product Code.	No. of Reactions	unitSize
15525033	100 Reactions	100 units
15535033	500 Reactions	500 units
15545033	5 x 500 Reactions	2500 units

Product Code. 15525033 Supplier Invitrogen™ Supplier No. 12351010

Description

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Benefits of Platinum SuperFi DNA Polymerase

• Exceptional >300X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult targets including those of sub-optimal purity or high GC content
• Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
• Amplification of samples with suboptimal purity

Applications

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Long PCR

Notes

• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Content And Storage: • Platinum SuperFi DNA Polymerase (50 μL at 2 U/μL)
  • 5X SuperFi Buffer (2 x 1.25 mL)
  • 5X SuperFi GC Enhancer (1.25 mL)
  
  Store at –5°C to –30°C.
• GC-Rich PCR Performance: High
• Polymerase: Platinum SuperFi DNA Polymerase
• Reaction Speed: Fast
• Quantity: 100 units
• Shipping Condition: Wet or Dry Ice
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 300X
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Overhang: Blunt
• Reaction Format: Separate Components
• Size (Final Product): 20 kb or less

Frequently Asked Questions (FAQs)

Can I use Taq polymerase to generate my gene of interest for directional TOPO cloning?

No, your gene of interest must be amplified with a proofreading polymerase such as Platinum SuperFi DNA Polymerase or AccuPrime Pfx DNA Polymerase that leaves blunt ends for directional TOPO cloning.

Which nucleotide analogues can be used with Platinum SuperFi DNA Polymerase?

Platinum SuperFi DNA Polymerase cannot read dUTP-derivatives or dITP in DNA templates, so the use of these analogues is not recommended.Platinum SuperFi DNA Polymerase can incorporate 7-deaza-dGTP and radiolabeled dNTPs. 

I am having difficulties amplifying long targets using Platinum SuperFi DNA Polymerase. What are your recommendations?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh and intact) and fresh primer solutions. Optimization steps to consider include decreasing denaturation and extension temperatures, lengthening extension times as recommended in the manual, and increasing template amounts.

Can Platinum SuperFi DNA Polymerase amplify AT-rich targets?

Yes. To improve amplification of AT-rich targets, we recommend reducing the extension temperature to 68 degrees C or add 5-15 mM Tetramethylammonium Chloride (TMAC).

Can Platinum SuperFi DNA Polymerase amplify GC-rich targets?

All Platinum SuperFi product formats are supplied with 5X SuperFi GC Enhancer which is optimized to improve amplification of GC-rich targets (recommended for use with targets containing >65% GC content)

Does SuperFi Green Buffer influence performance of Platinum SuperFi DNA Polymerase?

No. The colored buffer does not interfere with PCR performance and does not change enzyme features.

Can Platinum SuperFi DNA Polymerase be used for amplification of bisulfite-converted DNA?

No. Platinum SuperFi DNA Polymerase cannot read uracil in the template strand, therefore, it is not recommended for use with bisulfite-converted DNA.

Does Platinum SuperFi DNA Polymerase add the non-template dependent 3'-A overhang?

No. Platinum SuperFi DNA Polymerase generates blunt ended products.

What is the amplicon size limit using Platinum SuperFi DNA Polymerase?

Platinum SuperFi DNA Polymerase accurately amplifies long fragments (up to 20 kb) with high yields and specificity. Amplification of even larger fragment sizes up to 40 kb has been demonstrated, but may require additional optimization of reaction conditions and primer design.

Can protocols optimized for Taq DNA Polymerase, Platinum Taq DNA Polymerase High Fidelity, AccuPrime Taq DNA Polymerase High Fidelity, Platinum Pfx DNA Polymerase, or AccuPrime Pfx DNA Polymerase be directly applied to Platinum SuperFi DNA Polymerase?

Platinum SuperFi DNA Polymerase significantly differs from many other DNA polymerases, therefore annealing rules should be adjusted by using our Tm calculator (www.thermofisher.com/tmcalculator). Due to high processivity, Platinum SuperFi DNA Polymerase also has shorter cycling times than many other DNA polymerases.

For Research Use Only. Not for use in diagnostic procedures.