Macherey-Nagel™ Neutralization Buffer A3

Product Code. 12776553

Brand: Macherey-Nagel™ 740913.1

Description

Neutralization Buffer A3 to be used with NucleoSpin Plasmid, NucleoSpin Plasmid (NoLid), or NucleoSpin Plasmid EasyPure kits.

The eight-well format of the NucleoSpin™ miniprep columns is easily processed by vacuum filtration. The NucleoVac 96 vacuum manifold allows simultaneous processing of multiples of eight samples in less than 90min. The NucleoSpin™ Multi-8 Plus Plasmid kit also contains NucleoSpin™ Plasmid filter strips to clear bacterial lysates instead of time consuming centrifugation. The NucleoSpin™ Multi-8 Plus Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mLE. coliovernight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used. Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips. For further information on this product contact your local Customer Services.

• Silica-membrane technology
• Yield: up to 20μL sequencing grade plasmid DNA
• Elution volume: 75μL to 150μL
• Culture volume: 1mL to 5mL
• Binding capacity: 25μg
• Maximum plasmid size <15kb
• No use of organic solvents
• NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included Rapid small-scale preparation of high-copy plasmids fromE. coli.

Specifications

• Chemical Name or Material: Neutralization Buffer A3
• For Use With (Application): For Plasmid DNA Purification
• Quantity: 1000 mL

Safety and Handling

Product Identifier

• Buffer A3 (1000 mL)

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.

Supplemental information

• MIXTURE LIST-Contains : guanidium hydrochloride