Macherey-Nagel™ Elution Buffer AE

Product Code.	Quantity	unitSize
12716563	1000 mL	1000mL

Product Code. 12716563 Supplier Macherey-Nagel™ Supplier No. 740917.1

Description

Elution Buffer AE to be used with NucleoSpin Plasmid, NucleoSpin Plasmid (NoLid), or NucleoSpin Plasmid EasyPure kits.

NucleoSpin™ Plasmid kits feature a protocol that concludes with an elution step. Plasmid DNA Elution Buffer AE is a 5mM Tris/HCl solution with a pH of 8.5 for extracting pure plasmid DNA from the matrix of the NucleoSpin™ column under low ionic strength conditions.

Supplemental Elution Buffer AE is necessary when adapting the buffer volume to achieve a higher yield, which may be required for some downstream applications. NucleoSpin™ Plasmid kits include enough Elution Buffer AE to accommodate the standard protocol (typical recovery: 70–90%).

• Higher yield in general, esp. for larger constructs: Heat buffer, use up to 2x the buffer, incubate longer
• High yield: Perform two elution steps, which uses 2x the elution buffer, for a recovery of 90–100%
• High concentration: Use 60% volume of elution buffer for ∼130% concentration (max. yield: 80%)
• High yield and high concentration: Perform a longer incubation using 50% volume of elution buffer, then apply a standard elution step (yield: 85–100%, at a high concentration)

Specifications

• Chemical Name or Material: Tris–HCl solution
• CAS: 77-86-1
• Concentration: 5mM
• For Use With (Application): For Plasmid DNA Purification
• For Use With (Equipment): NucleoSpin™ Plasmid DNA kits
• pH: 8.5
• Quantity: 1000 mL