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Macherey Nagel™ Plasmid DNA purification, NucleoSpin™ , Concentrate Wash Buffer A4

Brand:  Macherey Nagel™ 740914.1

UNSPSC : 41105500

Code : 86

Additional Details : Weight : 0.39000kg

Quantity 200mL

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Product Code. 12796553

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Specifications

Description

The eight-well format of the NucleoSpin™ miniprep columns is easily processed by vacuum filtration. The NucleoVac 96 vacuum manifold allows simultaneous processing of multiples of eight samples in less than 90min. The NucleoSpin™ Multi-8 Plus Plasmid kit also contains NucleoSpin™ Plasmid filter strips to clear bacterial lysates instead of time consuming centrifugation. The NucleoSpin™ Multi-8 Plus Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mLE. coliovernight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used. Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips. For further information on this product contact your local Customer Services.
  • Silica-membrane technology
  • Yield: up to 20μL sequencing grade plasmid DNA
  • Elution volume: 75μL to 150μL
  • Culture volume: 1mL to 5mL
  • Binding capacity: 25μg
  • Maximum plasmid size <15kb
  • No use of organic solvents
  • NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included Rapid small-scale preparation of high-copy plasmids fromE. coli.
Description & Specifications

Specifications

Wash Buffer A4 Concentrate
For 1000mL Buffer A4
200mL
Documentation

Technical Documents