Macherey-Nagel™ Plasmid DNA purification, NucleoSpin™ , Concentrate Wash Buffer A4
Brand: Macherey-Nagel™ 740914
Code : 86
Additional Details : Weight : 0.06000kg
DescriptionThe eight-well format of the NucleoSpin™ miniprep columns is easily processed by vacuum filtration. The NucleoVac 96 vacuum manifold allows simultaneous processing of multiples of eight samples in less than 90min. The NucleoSpin™ Multi-8 Plus Plasmid kit also contains NucleoSpin™ Plasmid filter strips to clear bacterial lysates instead of time consuming centrifugation. The NucleoSpin™ Multi-8 Plus Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mLE. coliovernight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used. Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips. For further information on this product contact your local Customer Services.
- Silica-membrane technology
- Yield: up to 20μL sequencing grade plasmid DNA
- Elution volume: 75μL to 150μL
- Culture volume: 1mL to 5mL
- Binding capacity: 25μg
- Maximum plasmid size <15kb
- No use of organic solvents
- NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included Rapid small-scale preparation of high-copy plasmids fromE. coli.
|For 100mL Buffer A4|
|Wash Buffer A4 Concentrate|