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Macherey-Nagel™ Plasmid DNA purification, NucleoSpin™ , Concentrate Wash Buffer A4

Brand:  Macherey-Nagel™ 740914

Code : 86

Additional Details : Weight : 0.06000kg

Product Code. 10134761

  • £25.55 / 100mL

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The eight-well format of the NucleoSpin™ miniprep columns is easily processed by vacuum filtration. The NucleoVac 96 vacuum manifold allows simultaneous processing of multiples of eight samples in less than 90min. The NucleoSpin™ Multi-8 Plus Plasmid kit also contains NucleoSpin™ Plasmid filter strips to clear bacterial lysates instead of time consuming centrifugation. The NucleoSpin™ Multi-8 Plus Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mLE. coliovernight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used. Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips. For further information on this product contact your local Customer Services.
  • Silica-membrane technology
  • Yield: up to 20μL sequencing grade plasmid DNA
  • Elution volume: 75μL to 150μL
  • Culture volume: 1mL to 5mL
  • Binding capacity: 25μg
  • Maximum plasmid size <15kb
  • No use of organic solvents
  • NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included Rapid small-scale preparation of high-copy plasmids fromE. coli.


For 100mL Buffer A4
Wash Buffer A4 Concentrate

Technical Documents