Macherey Nagel™ Plasmid DNA purification, NucleoSpin™ , Concentrate Wash Buffer A4

Brand: Macherey Nagel™

Manufacturer Part Number: 740914

100 ML WASH BUFFER A4, 20ML CONCENTRATE TO MAKE100mL buffer Macherey-Nagel 100mL

UNSPSC: 41105500

Code: 86

Additional Details:
Additional Details: Weight: 0.06000kg

Quantity 20mL

Product Code. 10134761

Quantity Price
1 £24.64 / 100mL
Estimated Shipment
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Description and Specification


For Use With For 100mL Buffer A4
Quantity 20mL
Buffers Wash Buffer A4 Concentrate
Quantity 20mL

The eight-well format of the NucleoSpin™ miniprep columns is easily processed by vacuum filtration. The NucleoVac 96 vacuum manifold allows simultaneous processing of multiples of eight samples in less than 90min. The NucleoSpin™ Multi-8 Plus Plasmid kit also contains NucleoSpin™ Plasmid filter strips to clear bacterial lysates instead of time consuming centrifugation. The NucleoSpin™ Multi-8 Plus Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mLE. coliovernight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used. Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips. For further information on this product contact your local Customer Services.
  • Silica-membrane technology
  • Yield: up to 20μL sequencing grade plasmid DNA
  • Elution volume: 75μL to 150μL
  • Culture volume: 1mL to 5mL
  • Binding capacity: 25μg
  • Maximum plasmid size <15kb
  • No use of organic solvents
  • NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included Rapid small-scale preparation of high-copy plasmids fromE. coli.


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