Placental Alkaline Phosphatase, Biotin, clone: 8B6, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 13-9870-80

Product Code. 15527396

Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The exact physiological function of the alkaline phosphatases is not known. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.

Description: The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 (or 130 with 65 kDa) enzyme expressed by placental syncytiotrophoblastoid cells. This enzyme has slightly different enzyme kinetics from other phosphatases. PLAP can be found anchored to the plasma membrane in addition to the cytoplasm. Expression is found in several germ line cancers; ovarian and testicular. Expression is also present in human embryonic stem cells and bone marrow-derived mesenchymal stromal cells.This 8B6 antibody reacts with both the Regan and Nagao isozymes.Applications Reported: This 8B6 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue, and ELISA.Applications Tested: This 8B6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta with trypsin digestion. This antibody can be used at less than or equal to 20 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Placental Alkaline Phosphatase
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05187
• Gene Symbols: ALPP
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 250
• Content And Storage: Store at 2-8°C. Do not freeze.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: 8B6
• Conjugate: Biotin
• Gene: ALPP
• Gene Alias: FLJ11281, PLA2P, PLAA
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.