Invitrogen™ PKC alpha Monoclonal Antibody (MC5)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA1157

Product Code. 15366271

Description

Western blot analysis of MA1-157 detects an ~80 kDa protein in human, bovine, non-human primate, mouse, rat, dog and rabbit cells. In Jurkat, rat brain and MDCK cell lysates, a weak unknown band at ~140 kDa is also detected. MA1-157 shows specificity to PKC alpha and is non-reactive to other PKC family members.

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Specifications

• Antigen: PKC alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 30% glycerol, 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P00517, P04409, P05132, P05696, P10102, P17252, P20444, P27791, Q8MJ44
• Gene Symbols: PRKACA, PRKCA
• Immunogen: Purified bovine brain Protein Kinase C alpha.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Bovine, Canine, Human, Mouse, Monkey, Rabbit, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: MC5
• Conjugate: Unconjugated
• Gene: PRKACA
• Gene Alias: AAG6; aging-associated gene 6; AI875142; EC 2.7.11.13; FHC; FTH; FTHL6; im:7139045; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; Pkca; PKC-A; PKC-alpha; PKC-III; PKRCA; PLIF; PRKACA; Prkca; prkcaa; protein kinase C; protein kinase C alpha; protein kinase C alpha type; protein kinase C alpha type; protein kinase C, alpha, a; protein kinase C, alpha; protein kinase C, alpha, a; protein kinase c-alpha; si:dkey-8l19.1; Unknown (protein for IMAGE:7139045); unnamed protein product
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100008815, 100037720, 18747, 18750, 24680, 25636, 282001, 282322, 403556, 490904, 5566
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

Product Identifier

• PKC alpha Antibody (MA1-157) in ICC/IF

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : sodium chloride