Invitrogen™ PIP5K1A Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA529405

Product Code. 13403587

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (81%), Dog (95%), Pig (96%), Chicken (87%), Rhesus Monkey (97%), Chimpanzee (98%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PIP5K1A lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'disheveled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.

Specifications

• Antigen: PIP5K1A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Pip5k1a
• Gene Alias: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type I beta; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase type 1 alpha; phosphatidylinositol-4-phosphate 5-kinase type I alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type 1 beta; phosphatidylinositol-4-phosphate 5-kinase, type 1, alpha; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PI4P5K-I[a]; PI4P5KIbeta; PI51A; Pip5k1a; PIP5K1-alpha; Pip5k1b; PIP5KIalpha; Pipk5a; ptdIns(4)P-5-kinase 1 alpha
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18720, 8394
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.41 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P70182, Q99755
• Gene Symbols: Pip5k1a
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PIP5K1A. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG