Thermo Scientific™ Yeast DNA Extraction Kit

Product Code. 10473985

Brand: Thermo Scientific™ 78870

Description

Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.

Quickly and easily lyse yeast cells to release DNA for study with Thermo Scientific™ Pierce Yeast DNA Extraction Kit, which utilizes a Thermo Scientific γ-PER Reagent.

The kit provides greater yields than traditional methods of DNA isolation from yeast. Typically, extraction and purification of DNA from yeast are time-consuming and labor-intensive. The yeast proteinaceous cell wall is notoriously difficult to lyse and requires harsh treatments that are time-consuming and can damage the extracted DNA. The Yeast DNA Extraction Kit protocol requires less than one hour to complete, is effective without enzymatic treatment or glass beads, and yields little to no RNA contamination regardless of RNase presence. In studies with Saccharomyces cerevisiae, high yields of genomic and plasmid DNA are consistently obtained. DNA purified using this kit is suitable for PCR amplification, bacterial transformations, restriction digestions and hybridization applications.

Highlights:

• Eliminates need for glass beads or harsh enzyme treatments
• Yields DNA that is compatible with polymerase chain reaction (PCR) amplification
• Rapidly isolates plasmid DNA from S. cerevisiae suitable for transformation of E. coli
• Protocol is easily scalable for use with single colonies or 500mL cultures

Includes:

Thermo Scientific γ-PER Yeast Protein Extraction Reagent, DNA releasing reagents A and B, and protein removal reagent

Specifications

• Content And Storage: Sufficient For: 50 purifications from 10 mL cultures
  • Y-PER Yeast Protein Extraction Reagent, 25 mL
  • DNA Releasing Agent A, 20 mL
  • DNA Releasing Agent B, 20 mL
  • Protein Removal Reagent, 10 mL
  
  Store at 4°C. Precipitates may form; gently warm at 37°C for 1–5 minutes.
• Sample Type: Yeast
• For Use With (Application): PCR, Sequencing, Transformation
• High-throughput Compatibility: Not High-throughput Compatible
• Product Type: Yeast DNA Extraction Kit
• Quantity: 75 mL
• Target: Genomic DNA

Safety and Handling

Product Identifier

• Yeast DNA Extraction Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P264-Wash thoroughly after handling.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : lithium chloride, sodium dodecyl, trometamol, sodium chloride, sodium chloride,disodium hydrogenort

For Research Use Only. Not for use in diagnostic procedures.