Thermo Scientific™ Pierce™ Trifluoracetic Acid (TFA)

Bottles of 99.5%-pure TFA (MW 114.02, CAS 76-05-01) liquid (sold by weight); for HPLC use as an ion-pairing agent in reverse-phase peptide separations.

Brand: Thermo Scientific™

Manufacturer Part Number: 85183

50mL TFA (Trifluoroacetic Acid), LC-MS grade

UNSPSC: 41116105

Code: PN

Additional Details:
Additional Details: CAS Number: 76-05-1 Weight: 0.45360kg Transport: UN number : 2699 Chem class : 1a Pack group : I

Product Code. 13464279

Quantity Price
1 £ 125.0 / 50mL
EU Stock 32
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Description and Specification


For Use With LC-MS
Quantity 50mL
Standard Components Trifluoroacetic acid
Formulation Clear, colorless liquid of TFA (at least 99.5% pure; zero aldehyde; low water; low absorbance)
Item Name Trifluoroacetic Acid (TFA), LC-MS Grade

For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptide that would otherwise overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.


  • Purity: >99.5% TFA and exceptional clarity allows sensitive, nondestructive peptide detection at low UV wavelengths
  • High-performance packaging: Packaged under nitrogen in amber glass with protective TFE-lined fluorocarbon caps to ensure TFA integrity
  • Choice of formats for convenience: 1mL ampules can prepare 1L of 0.1% v/v TFA solution for the mobile phase in reverse-phase chromatography in moments

Ordering Information:

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