Thermo Scientific™ Pierce™ Trifluoracetic Acid (TFA)

Bottles of 99.5%-pure TFA (MW 114.02, CAS 76-05-01) liquid (sold by weight); for HPLC use as an ion-pairing agent in reverse-phase peptide separations.

Brand: Thermo Scientific™

Manufacturer Part Number: TS-28901

UNSPSC: 41120000

Code: A0

Additional Details:
Additional Details: Weight: 0.55000kg Transport: UN number : 2699 Chem class : 1a Pack group : I

Disclaimers: Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Product Code. 13416806

Quantity Price
1 £ 319.0 / 500mL
Estimated Shipment
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Description and Specification


Storage 20 to 25°C
Item Name Trifluoroacetic Acid (TFA), Sequencing grade
Formulation Liquid
For Use With Protein biology, protein mass spectrometry analysis, calibration solutions, standards and solvents for mass spectrometry, protein/peptide sequencing, protein/peptide solubilizing agent, amino acid analysis, making 0.1% solutions of trifluoroacetic acid (w/v vs. v/v)
Description Trifluoroacetic Acid (TFA), 500mL, Sequencing Grade, Liquid, Ion Pairing Reagent, High Purity and Exceptional Clarity, High-Performance Packaging, Sequencing Grade, Economical Convenience, Ideal for use in Protein/Peptide Sequencing, Sequencing Grade, Solid-phase Peptide Synthesis
Standard Concentration 98 to 100%
Quantity 500mL
Standard Components Trifluoroacetic acid

For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptide that would otherwise overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.


  • Purity: >99.5% TFA and exceptional clarity allows sensitive, nondestructive peptide detection at low UV wavelengths
  • High-performance packaging: Packaged under nitrogen in amber glass with protective TFE-lined fluorocarbon caps to ensure TFA integrity
  • Choice of formats for convenience: 1mL ampules can prepare 1L of 0.1% v/v TFA solution for the mobile phase in reverse-phase chromatography in moments

Ordering Information:

X in the ordering table indicates that hazardous shipping charges apply.