Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Brand: Thermo Scientific™

Manufacturer Part Number: 34028


UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.25000kg

Product Code. 10647894

Quantity Price
1 £143.00 / 250mL
EU Stock 128
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Description and Specification


Description Pierce 1-Step Ultra TMB-ELISA Substrate Solution
Formulation Proprietary ready-to-use solution of 3,3',5,5'-tetramethylbenzidine
Quantity 250mL
Sufficient For 250mL of 1X substrate (25 microplate assays)

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.


  • Ready-to-use solutions
  • No hydrogen peroxide required
  • No filtering required
  • Noncarcinogenic
  • Various sensitivities to suit any assay

1-Step TMB Substrates

  • Ready-to-use, one-component substrates
  • Do not require hydrogen peroxide
  • Noncarcinogenic
  • Do not contain DMF or DMSO
  • Available in three formulations that vary by sensitivity

TMB Substrate Kit

  • More sensitive than OPD in endpoint ELISAs
  • Easy to use
  • Provides results in seconds


TMB Substrate Kit (PI-34021): Peroxidase substrate, 200mL; Peroxide solution, 200mL


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