Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 34021

400 ML TMB SUBSTRATE KIT 400ML STORE IN FRIDGE AT4C

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg



Product Code. 10076433

Quantity Price
1 £ 148.0 / 400mL
EU Stock 49
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Description and Specification

Specification

Description

Substrate Solution, 200mL Stable Peroxide Solution

Formulation Two-component kit with substrate and peroxide solutions
Kit Contents

Peroxide Solution, 200mL

Quantity 400mL kit
Sufficient For 400mL of 1X substrate (40 microplate assays)

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.

Highlights:

  • Ready-to-use solutions
  • No hydrogen peroxide required
  • No filtering required
  • Noncarcinogenic
  • Various sensitivities to suit any assay

1-Step TMB Substrates

  • Ready-to-use, one-component substrates
  • Do not require hydrogen peroxide
  • Noncarcinogenic
  • Do not contain DMF or DMSO
  • Available in three formulations that vary by sensitivity

TMB Substrate Kit

  • More sensitive than OPD in endpoint ELISAs
  • Easy to use
  • Provides results in seconds

Includes:

TMB Substrate Kit (PI-34021): Peroxidase substrate, 200mL; Peroxide solution, 200mL

Documentation

Technical documents