Thermo Scientific™ Pierce™ Thiophilic Adsorbent Resin and Kit

Preferentially bind and purify immunoglobulins from various sources including mouse, rat, rabbit, goat and human with this non-proteinaceous immunosorbent resin.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 44916

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg Transport: UN number : 2811 Chem class : 1 Pack group : III



Product Code. 10097143

Quantity Price
1 £ 463.0 / 1 set
Estimated Shipment date
from Supplier 11-04-2017
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Description and Specification

Specification

Formulation Multi-component kit
Kit Contents Binding Buffer, 1L
Sufficient For Purifying up to 60mg immunoglobulin per column
Format Drip column
Description Pierce Thiophilic Adsorption Kit
Target IgG
Item Description Kit with 3mL columns; 4-column kit
Support Type Agarose
Quantity 4 column kit

Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity. The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers.

Highlights:

  • High capacity – gently bind and purify approx. 20mg polyclonal antibody per milliliter of adsorbent resin

  • Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY

  • Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps

  • Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody

Includes:

Kit contains four Thiophilic Adsorbent Columns (3mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories

Recommended for:

Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications