1L of 1X buffer with appropriate peroxidase substrate
The Stable Peroxide Substrate Buffer is an ideal formulation for researchers who prefer to make their own horseradish peroxidase (HRP) substrates. The substrate is easily prepared by adding the chosen chromogen to a 1X solution of the Stable Peroxide Substrate Buffer, which supplies the needed peroxide and buffer condition to obtain optimum and consistent results. This buffer is provided at a 10X concentrate, which allows a shelf life of up to one year. The Diethanolamine Buffer is a 5X stock solution (5.1M, pH 9.8) that provides optimal conditions for development of alkaline phosphatase reactions, such as with PNPP (Part No. 34045, 34047).