Thermo Scientific™ Pierce™ Strong Ion Exchange Spin Columns

Perform rapid protein fractionation and sample preparation based on charge differences using these strong cation and anion exchange spin columns.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 90010

X24 STRONG ANION EXCHANGE SPIN CO24/ Pkg

UNSPSC: 41115711

Code: PN

Additional Details:
Additional Details: Weight: 3.85550kg



Product Code. 10311514

Quantity Price
1 £ 109.0 / Pack of 24
Estimated Shipment date
from Supplier 15-12-2016
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Description and Specification

Specification

Description Pierce Strong Anion Exchange Spin Columns, Mini
Formulation Polypropylene microcentrifuge columns with bottom ion exchange filter membrane; Microcentrifuge collection tubes
Quantity 24 columns
Sufficient For 24 samples, each ≤ 4mg and ≤ 0.5mL
Capacity (Metric) 0.5mL
Item Description Anion Columns, Mini size (4mg, 500uL capacity); 24 columns

Thermo Scientific Pierce Strong Cation and Anion Ion Exchange Columns use adsorbent chromatographic matrices to fractionate proteins based on charge differences. The adsorbent membranes and column structures are optimized for rapid sample processing by centrifugation. The membrane-based adsorbents have a highly porous structure (pores larger than 3000nm), providing proteins unhindered access to the charged affinity ligands. These features provide for high efficiencies and flow rates when fractionating large biomolecules with small diffusivities. Columns are available in mini (0.5mL microcentrifuge) and maxi (20mL centrifuge) sizes in both anionic and cationic formats.

Pierce Ion Exchange Spin Columns replace time-consuming, tedious and expensive chromatographic methods for many protein applications. The centrifuge-purification format based on membrane adsorbents allows convenient high-yield processing of multiple samples in less than 20 minutes. The purified protein is compatible with downstream applications such as sample fractionation for 1D and 2D SDS-PAGE, X-ray crystallization and NMR spectroscopy.

Highlights:

  • Fast and simple – membrane-based spin format eliminates column packing required with traditional resin methods

  • Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel

  • Durable and robust – spin columns and integrated adsorbent membranes do not crack or fail when run dry

  • Small bed volume – adsorbent membrane has a very small effective bed volume, which minimizes sample loss and dilution effects with small-volume samples

  • Anion and cation formats – choose either negatively charged or positively charged ion exchange columns

  • Two column sizes – choose either Mini size (4mg, 0.5mL capacity) or Maxi size (80mg, 20mL capacity) columns

Includes:

Ready-to-use columns and two collection tubes per column

Recommended for:

Pre-fractionation of proteins in lysate; Scouting purification conditions for new protein preparation protocols; Removal of endotoxins from monoclonal antibodies; Polishing His-tagged proteins after metal chelate chromatography; Purification of antibodies from serum, ascites or tissue culture supernatant; Removal of detergent from protein solutions; Sample preparation before 1D or 2D PAGE; Purification of phosphopeptides before MS analysis.

Documentation

Technical documents