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Description
Thermo Scientific Pierce Streptavidin is a purified unconjugated recombinant protein form.
Features of Streptavidin:
- Recombinant streptavidin—high-quality protein, 53kDa, pI = 6.8 to 7.5
- Pure protein —choose pure, unconjugated protein for biotin blocking and controls
The purified recombinant Streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin is supplied as lyophilized in phosphate-based buffers for immediate reconstitution with water.
Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
Related Products
- Pierce™ Streptavidin, Horseradish Peroxidase Conjugated (Cat. No. 21126)
- Pierce™ Streptavidin, Alkaline Phosphatase Conjugated (Cat. No. 21324)
- Pierce™ Streptavidin, Maleimide-Activated (Cat. No. 21102)
- Pierce™ Streptavidin, Hydrazide-Activated (Cat. No. 21120)
Specifications
Specifications
| Conjugate | Unconjugated |
| Quantity | 5 mg |
| Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C. |
| Form | Solid |
| Product Line | Pierce |
| Product Type | Streptavidin |
| Target Molecule | Biotin |
| Label or Dye | Unlabeled |
For Research Use Only. Not for use in diagnostic procedures.
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