Thermo Scientific™ Pierce™ Standard Binding NeutrAvidin Coated Plates

Capture biotinylated antibodies or probes for ELISA and other target-specific assays with pre-blocked NeutrAvidin protein-coated plates.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 15400

X5 Microplate Pierce Reacti-Bind 384 well NeutrAviPlates, 5 Plates

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10165713

Quantity Price
1 £ 257.0 / Pack of 5
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Binding Target Biotin
Description Pierce NeutrAvidin Coated Plates, Clear, 384-Well
Formulation Clear, 384-well plates, coated with 50uL of NeutrAvidin Protein and blocked with 100uL of SuperBlock Blocking Buffer
Quantity 5 plates
Sufficient For Binding approx. 10 pmol biotin per well

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Thermo Scientific NeutrAvidin™ Protein is avidin that has been deglycosylated to prevent nonspecific lectin-binding. NeutrAvidin Protein has a near-neutral pI (6.3), resulting in lower nonspecific interactions in certain applications compared with streptavidin. NeutrAvidin Protein has an additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells. Pierce NeutrAvidin Coated Plates are available in clear, white and black plates for use with colorimetric, chemiluminescent and fluorescent detection methods, respectively.

Highlights:

  • Easy and gentle immobilization of biotin-containing conjugates
  • Lowest nonspecific binding properties of all biotin-binding proteins
  • Thermo Scientific NeutrAvidin Biotin-Binding Protein has no carbohydrate and an isoelectric point of 6.3
  • No denaturing of the protein component of a conjugate upon binding to the plate
  • Ideal for binding small hydrophilic molecules (e.g., peptides) that typically exhibit poor binding directly to polystyrene
  • Pre-blocked with your choice of Thermo Scientific Blocker BSA or SuperBlock Blocking Buffer
  • Available in 96- and 384-well formats

Documentation

Technical documents