Thermo Scientific™ Sodium Deoxycholate Detergent

Produktkod.	Quantity	Förpackningsstorlek
11864141	5 g	5g
11874141	25 g	25g

Produktkod. 11864141 Leverantör Thermo Scientific™ Leverantörsnummer 89904

Beskrivning

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Thermo Scientific™ Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.

Features of Sodium Deoxycholate:

• Popular anionic, bile-acid detergent for many laboratory uses
• Effective in disrupting and dissociating many types of protein interaction
• Can be removed from solution by dialysis
• Useful for elution or regeneration of certain kinds of affinity columns
• High-purity compound with low UV absorptivity

Properties of Sodium Deoxycholate:

• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt
• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt
• Molecular Weight: 414.6 g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 5 (average)
• Micelle Molecular Weight: 2000 g (average)
• Critical Micelle Concentration (CMC): 2 to 6 mM (0.083 to 0.249%, w/v)
• Cloud Point: Unknown
• Dialyzable: Yes

Specifications for Sodium Deoxycholate (Part No. 89904, 89905):

• Formula: C₂₄H₃₉O₄Na
• Molecular Weight: 414.6 g
• Purity (by HPLC): ≥98%
• Absorbance (1% Detergent Solution): 340 nm <0.02; 280 nm <0.04; 260 nm <0.06
• pH (1% Solution): 5 to 9
• Solubility (in water at 20°C): ≥5%

Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.

The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ∼2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.

Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.

Related Products

Surfact-Amps™ Detergent Sampler (Cat. No. 28340)

Specifikationer

• Product Type: Detergent
• Content And Storage: Store at -20°C
• Form: Powder
• Reagent Type: Detergent (Pure)
• Quantity: 5 g

Säkerhet och hantering

Produktbeteckning

• Sodium Deoxycholate

Signalord

• Warning

Riskkategorier

• Acute toxicity Category 4
• Specific target organ toxicity Category 3

Faroangivelser

• H302-Harmful if swallowed.
• H335-May cause respiratory irritation.

Skyddsangivelse

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P264-Wash hands thoroughly after handling.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Kompletterande information

• MIXTURE LIST-Contain: sodium 3-droxy-5-oate

For Research Use Only. Not for use in diagnostic procedures.