Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits

Product Code.	Product Type	unitSize
11688833	Protein Quantitation Kit - DMEM:F12	Each
10220204	Protein Quantitation Kit - RPMI 1640	1 set

Product Code. 11688833 Supplier Thermo Scientific™ Supplier No. 88439

Description

Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.

Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Efficient – 100% label incorporation into proteins of living cells

• Reproducible – eliminates intra-experimental variability caused by differential sample preparation

• Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling

• Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others

• Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

• High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation

Recommended for:

Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment

Specifications

• Formulation: Multi-component kit with heavy and light L-Lysine and DMEM:F12 Media
• Kit Contents: L-Lysine-2HCl, 50mg, Dialyzed FBS, 2 x 50mL, DMEM:F12 Media for SILAC, 2 x 500mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6, 50mg
• Product Type: Protein Quantitation Kit - DMEM:F12
• Sufficient For: One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)
• Quantity: 1 Use Kit
• For Use With (Application): SILAC experiments (stable isotope labeling with amino acids in cell culture)