Thermo Scientific™ Pierce™ SILAC Media and Sera

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88425

135 GR POWDERED DMEM FOR SILAC, MAKES 10L MEDIUM135g Store in Fridge at 4C

UNSPSC: 41115404

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12172410

Quantity Price
1 £ 239.0 / 135g
Estimated Shipment date
from Supplier 06-03-2017
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Description and Specification

Specification

Formulation DMEM media minus L-Leucine, L-Lysine and L-Arginine
For Use With SILAC experiments (stable isotope labeling with amino acids in cell culture)
Sufficient For 10L media for SILAC experiments
Description Powdered DMEM Media for SILAC
Quantity 135g
For Use With (Application) SILAC experiments (stable isotope labeling with amino acids in cell culture)

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

  • Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

  • Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation