Thermo Scientific™ Pierce™ SDS-PAGE Sample Prep Kit

Product Code.	Quantity	unitSize
10617514	50 reactions	1 set

Product Code. 10617514 Supplier Thermo Scientific™ Supplier No. 89888

Description

Quickly and easily remove high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis.

Thermo Scientific™ Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Highlights:

• Eliminates gel artifacts caused by incompatible contaminants – removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis

• Compatible with the BCA protein assay – allows protein quantitation of the processed sample before gel-loading

• Enriches dilute protein solutions – concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes

• Powerful and flexible – process 2 to 350μL of sample containing up to 70μg of protein; recover 75 to 85% of the total protein in 50μL of elution buffer

• Convenient, complete kit – includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

  • Includes:

    PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

Specifications

• Content And Storage: Upon receipt store kit at room temperature.
• Buffer: Sample Loading Buffers
• Gel Type: SDS-PAGE
• Gel Compatibility: SDS-PAGE Gels
• Quantity: 50 reactions
• Product Line: Pierce
• Product Type: SDS-PAGE Sample Prep Kit

Safety and Handling

Product Identifier

• Pierce SDS-PAGE Sample Prep Kit

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity after repeated exposure Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.
• H373-May cause damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P314-Get medical advice/attention if you feel unwell.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contain: Kieselguhr, calcined

For Research Use Only. Not for use in diagnostic procedures.