Thermo Scientific™ Pierce™ S-Nitrosylation Western Blot Kit

Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with a complete, easy-to-use kit.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 90105

S-NITROSYLATION WESTERN BLOT KIT, 40 REACTIONS

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.12000kg Transport: UN number : 3334 Chem class : 1 Pack group : III



Product Code. 13464199

Quantity Price
1 £ 315.0 / 0.20mL
EU Stock 4
Add to basket


Description and Specification

Specification

Description Pierce S-Nitrosylation Western Blot Kit
Formulation Multi-component kit
Kit Contents Anti-TMT Antibody, 100µL
Quantity 40 rxn kit
Sufficient For 40 labeling reactions of 100µg each

The Pierce S-Nitrosylation Western Blot Kit contains a cell lysis/reaction buffer, a sulfhydryl–reactive blocking agent, a reducing agent, a labeling agent and a detection monoclonal antibody. Each kit has enough reagents to label 40 samples of 100μg each. Similar to the traditional S–nitrosylation switch assay, unmodified cysteines are first blocked using a sulfhydryl–reactive compound (MMTS). S–nitrosylated cysteines are then selectively reduced with ascorbate in HENS Buffer for specific labeling with iodoTMTzero™ Reagents, which irreversibly bind to the cysteine thiol that was S–nitrosylated. Detection of the TMT™ reagent–modified proteins is facilitated using an anti–TMT antibody.

Highlights:

  • Specific – optimized labeling reactions switch S–nitrosocysteine post translational modifications with iodoTMT Reagent for easy detection by Western blot using anti–TMT antibody

  • Better signal-to-noise – uses a non–biological iodoTMT Reagent for labeling instead of HPDP–biotin, resulting in less background during Western blot detection

  • Workflow-compatible – labeling method is compatible with alternative methods of analysis, including enrichment via anti–TMT resin and multiplex quantitation by mass spectrometry

  • Convenient – all needed reagents included one kit

Includes:

Cysteine–blocking reagent, reducing agent, reaction buffer, iodoTMT™ labeling reagent, and anti-TMT antibody