Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 89900

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: Weight: 2.72160kg



Product Code. 10017003

Quantity Price
1 £ 64.0 / 100mL
EU Stock 121
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Description and Specification

Specification

Formulation 25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS
Source Material Cultured mammalian cells
Sufficient For Approx. 5 million cells per 1mL of reagent
Format Liquid
Description Pierce RIPA Buffer
Quantity 100mL

This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.

Highlights:

  • Convenient – ready-to-use solution

  • Flexible – compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification

  • Versatile – enables extraction of cytoplasmic, membrane and nuclear proteins

  • Disclosed formulation – contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues

Documentation

Technical documents