Thermo Scientific™ Pierce™ Recombinant Protein G and Conjugates

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 31499

IMMUNOPURE PROT G,PEROX CONJ 0,5MG

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.80000kg



Product Code. 11899150

Quantity Price
1 £ 135.0 / 500µg
EU Stock 10
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Description and Specification

Specification

Description Pierce Recombinant Protein G, Peroxidase Conjugated
Formulation Lyophilized powder from protein in buffered solution
Quantity 0.5mg

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA