Thermo Scientific Pierce™ Purified Recombinant Protein A and Conjugates

Detect rabbit and human antibodies, especially IgG isotypes, in Western blot, ELISA and other immunoassays with this purified and peroxidase-conjugated recombinant Protein A.

Overview
Brand: Thermo Scientific

Manufacturer Part Number: 77674

500MG PIERCE RECOMBINANT PROTEIN A

UNSPSC: 41105300

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15275683

Quantity Price
1 £ 1496.0 / 500mg
Estimated Shipment date
from Supplier 16-12-2016
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Description and Specification

Specification

Formulation Salt-free powder
Quantity 500mg

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. coli and functions essentially the same as native Protein A. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6).

Highlights:

  • Contains four Fc-binding domains per protein
  • Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
  • Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies