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Beschreibung
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.
Features of Protein-Free Blocking Buffer include:
- Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
- Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
- Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
- High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.
Spezifikation
Spezifikation
| Chemical Name or Material | Protein-Free (TBS) |
| Concentration | 1X |
| For Use With (Application) | ELISA |
| Physical Form | Liquid |
| Quantity | 100 mL |
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