Thermo Scientific™ Pierce™ Protein A, G, and L Coated Plates

Capture antibody complexes with these alternatives to direct, passive adsorption for immobilizing antibodies for ELISA and other plate-based assays.

Brand:  Thermo Scientific™ 15133

Product Code. 10063883

Description

These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G or Protein L). They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Highlights:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5pmol of antibody per well (based on tests with rabbit IgG)

Protein A/G Coated Microplates:

• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

Protein A Coated Microplates:

• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Protein G Coated Microplates:

• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Protein L Coated Microplates:

• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

Specifications

• Color: Clear
• Quantity: 5 Plates
• Binding Target: IgG
• Sufficient For: Binding approx. 2pmol rabbit IgG per well

• Format: 8-Well Strips
• Surface Coating: Protein G
• Formulation: Clear, 8-well strip plates, coated with 100uL of Protein G and blocked with 200uL of SuperBlock Blocking Buffer