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Thermo Scientific™ Pierce™ Protein A, G, and L Coated Plates
Capture antibody complexes with these alternatives to direct, passive adsorption for immobilizing antibodies for ELISA and other plate-based assays.
Brand: Thermo Scientific™ 15133
Code : PN
Additional Details : Weight : 0.10000kg
Description
These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G or Protein L). They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.Highlights:
- Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
- Orient antibodies for maximum antigen-binding capacity
- Immobilize antibodies for plate assays without prior purification
- Ensure minimal variation (<5% well-to-well) from consistent coating
- Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
- Bind 2 to 5pmol of antibody per well (based on tests with rabbit IgG)
Protein A/G Coated Microplates:
- Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
- Binds to most IgG species because it combines the specificities of Protein A and Protein G
- Binds to Fc region of antibodies, ensuring optimal orientation
- Binds well using a wide range of pH
Protein A Coated Microplates:
- Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
- Binds strongly to mouse IgG2a, IgG2b and IgG3
- Binds to Fc region of antibodies for optimal orientation
Protein G Coated Microplates:
- Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
- Binds only to IgG—no cross-reactivity with other antibody classes
- Binds to Fc region of antibodies for optimal orientation
Protein L Coated Microplates:
- Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
- Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
- Binds to Fab fragments and ScFv
- Does not bind bovine, goat or sheep immunoglobulin
- Binds weakly to rabbit immunoglobulin
- Pre-blocked to reduce nonspecific binding
Specifications
Clear | |
5 Plates | |
IgG | |
Binding approx. 2pmol rabbit IgG per well |
8-Well Strips | |
Protein G | |
Clear, 8-well strip plates, coated with 100uL of Protein G and blocked with 200uL of SuperBlock Blocking Buffer |