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Description
Thermo Scientific Pierce PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.
PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid. This protein stain delivers a dynamic range of 5 to 500 ng, which is approximately 10 times more sensitive than traditional coomassie R-250-based dyes. Furthermore, PageBlue Protein Staining Solution is economical because it can be reused up to three times without any loss in sensitivity.
Highlights:
- Easy-to-use – simply soak the gel in the ready-to-use solution and observe stained bands without destaining
- Fast – 25 to 40 minute protocol
- Safe – does not contain methanol or acetic acid
- Dynamic range – linear dynamic range of 5 to 500 ng
- Economical – can be reused up to 3 times
Recommended for:
- Visualize and quantify proteins separated in 1D, 2D and IEF polyacrylamide gels
- Stain proteins after transfer onto PVDF membranes
- Stain gels prior to subsequent silver staining or mass spectrometry analysis
Specifications
Specifications
| Content And Storage | Upon receipt store product at room temperature. Product shipped at ambient temperature |
| Detection Location | In-Blot Detection, In-Gel Detection |
| Detection Method | Colorimetric |
| Label or Dye | Coomassie |
| Quantity | 1 L |
| Target Molecule | Protein |
| Product Line | PageBlue |
| Product Type | Protein Staining Solution |
Safety and Handling
- PageBlue Protein Staining Solution, 1 L
- Danger
- Flammable liquid Category 3
- Serious eye damage/eye irritation Category 1
- H226-Flammable liquid and vapour.
- H318-Causes serious eye damage.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P233-Keep container tightly closed.
- P243-Take precautionary measures against static discharge.
- P240-Ground/bond container and receiving equipment.
- P370+P378-In case of fire: Use to extinguish.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P310-Immediately call a POISON CENTER/doctor/.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501-Dispose of contents/container to .
- MIXTURE LIST-Contain: Isopropyl alcohol, Phosphoric acid
For Research Use Only. Not for use in diagnostic procedures.
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