Thermo Scientific™ Pierce™ Nickel Coated Plates, Black, 96-Well
Bind His-tagged fusion proteins for ELISA and other plate-based assays to study interactions of expressed recombinant proteins.
Brand: Thermo Scientific™ 15242
Code : PN
Additional Details : Weight : 0.10000kg
DescriptionThese Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
- Detergents used to lyse cells do not inhibit binding to Ni2+coated plates as they do with plain polystyrene
- The detection limit is 1ng of histidine fusion protein
- Better binding for more sensitive assays compared to other commercially available nickel-activated plates
|Standard Capacity, Affinity|
|Detection Plate, Immunoassay, ELISA|
|Pierce Nickel Coated Plates, White, 96-Well|
|Bovine Serum Albumin (BSA)|
|Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.|
|Divalent Nickel, Metal Chelate|