Thermo Scientific™ Pierce™ Nickel Coated Plates

Bind His-tagged fusion proteins for ELISA and other plate-based assays to study interactions of expressed recombinant proteins.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 15242

X5 Microplate Pierce HisGrab 96 well nickel coatedPlates

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10033983

Quantity Price
1 £ 187.0 / Pack of 5
Estimated Shipment date
from Supplier 09-01-2017
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Description and Specification

Specification

Binding Target His tag
Description Pierce Nickel Coated Plates, White, 96-Well
Formulation White, 96-well plates, coated with 200uL of Nickel ion and blocked with 200uL of BSA
Quantity 5 plates
Sufficient For Binding approx. 9pmol His-tagged protein per well

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Highlights:

  • Detergents used to lyse cells do not inhibit binding to Ni2+coated plates as they do with plain polystyrene
  • The detection limit is 1ng of histidine fusion protein
  • Better binding for more sensitive assays compared to other commercially available nickel-activated plates

Documentation

Technical documents