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Pierce™ NeutrAvidin™ Protein and Conjugates

Purified NeutrAvidin protein for Western blot, ELISA or other biotin-based detection methods.

Brand:  Thermo Scientific Pierce 31007

Code : PN

Additional Details : CAS Number : 3275-24-9 Weight : 0.80000kg

Product Code. 11855855

  • £109.00 / 5mg


Estimated Shipment
07-12-2017
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Description

Description

NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.

Highlights:

  • Near-neutral isoelectric point – pI equals 6.3, more neutral than native avidin

  • Nearly devoid of glycosylation – decreased possibility of lectin binding compared to native avidin

  • No RYD recognition sequence – no known off-target binding domains like streptavidin

  • Affordable – significantly less expensive than streptavidin

  • Available in multiple formats – choose purified NeutrAvidin Protein, prepared HRP and AP conjugates or activated forms for a variety of techniques

Specifications

Specifications

Pierce NeutrAvidin Protein, Maleimide Activated
Lyophilized powder from activated protein in buffered saline solution
5mg
Conjugation to sulfhydryl groups of approx. 5mg of antibody (150kDa) or molar equivalent
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