Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit

Product Code.	Quantity	unitSize
10781775	2.5 mL kit	1 set

Product Code. 10781775 Supplier Thermo Scientific™ Supplier No. 44980

Description

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Features of the Mouse IgG1 Fab and F(ab')2 Preparation Kit:

• High capacity—kit provides for fragmenting 0.25 to 4 mg IgG
• Complete—kit includes all reagents needed to prepare and purify antibody fragments
• Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
• Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass

Related Products

• Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit (Cat. No. 44680)
• Pierce™ F(ab')2 Preparation Kit (Cat. No. 44988)
• Pierce™ Fab Preparation Kit (Cat. No. 44985)

Specifications

• Quantity: 2.5 mL kit
• Product Line: Pierce
• Kit Contents: Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit
• Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG
  • Pierce Ficin Agarose, 2.5 mL
  • Cysteine-HCl, 1 g
  • Mouse IgG1 Digestion Buffer, 120 mL
  • NAb Protein A Plus Spin Column, 1 mL, 1 column
  • Protein A Binding Buffer, 120 mL
  • IgG Elution Buffer, 120 mL
  • Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
  • Spin Columns, 0.8 mL, 10 columns
  • Microcentrifuge tubes, 2 mL, 30 tubes
  
  Store at 4–8°C.

Sikkerhed og håndtering

Produktidentifikator

• Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit

Signalord

• Warning

Farekategori

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Faresætning

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Sikkerhedssætning

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplerende oplysninger

• MIXTURE LIST-Contain: Sodium chloride, L-(+)-Cysteine hydrochloride monohydrate

For Research Use Only. Not for use in diagnostic procedures.