Thermo Scientific™ Pierce™ Monomeric Avidin Resins

Reversibly bind biotinylated proteins and other ligands with monomers of avidin covalently attached as individual subunits onto beaded resins.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 20227

1 SET IMMOBILISED MONOMERIC AVIDIN KIT 00

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg Transport: UN number : 2811 Chem class : 1 Pack group : II



Product Code. 10475695

Quantity Price
1 £ 432.0 / 1 set
EU Stock 1
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  • Description and Specification
  • Documentation
  • Accessories and Spare Parts
Description and Specification

Specification

Description Pierce Monomeric Avidin Agarose Kit, 2mL
Formulation Multi-component kit with 1 x 2mL resin column
Kit Contents Biotin Binding and Elution Buffers, 200mL
Quantity 1 column kit
Sufficient For Binding >/= 2.4mg biotinylated BSA per column
Support Type Agarose

Pierce Monomeric Avidin Kit and Resins are unique among biotin-binding supports in allowing non-denaturing affinity purification of biotinylated molecules. The immobilized monomeric avidin protein binds biotin with high specificity and moderate affinity, allowing non-biotin molecules to be washed away and then the bound biotin-labeled molecules to be competitively eluted using 2mM biotin in phosphate buffered saline (PBS). This technique provides the gentlest elution conditions for biotinylated protein purification and avoids the contamination and other problems associated with traditional avidin and streptavidin methods. The monomeric avidin column can then be regenerated by using 0.1M glycine to strip the column of residual bound biotin without losing the ability to bind another biotinylated sample.

With typical avidin or streptavidin, the biotin-binding affinity (Kd = 10-15M) is so great that purification with these traditional media require denaturing conditions for elution, such as 8M Guanidine·HCl at pH 1.5 or boiling in reducing SDS-PAGE sample loading buffer. In addition to their adverse effects on the biotinylated protein of interest, such harsh elution conditions typically cause extraneous proteins (i.e., the avidin or streptavidin subunits) to strip from the resin and co-elute with the desired purification product. By contrast, when avidin is coupled to a resin (e.g., crosslinked beaded agarose or UltraLink Resin) as the subunit monomer, its specificity for biotin is retained, but its biotin-binding affinity decreases to a level (Kd = 10-8M ) that is conducive to subsequent elution.

Highlights:

  • Non-denaturing – purifies biotinylated products using mild elution conditions (2mM free biotin)

  • Reusable – Monomeric Avidin Agarose can be regenerated and reused at least 10 times, with only a marginal loss in biotin binding capacity (approximately 2.5% decrease per regeneration)

  • Specific – retains biotin-binding specificity and low nonspecific binding of native avidin

  • Flexible – bind samples in a variety of physiological buffers and elute either with 0.1M glycine or by competition with 2mM biotin

  • Good binding capacity – greater than 1.2mg biotinylated BSA/mL of resin

  • Convenient – purchase standalone agarose or UltraLink™ Resins or a complete purification kit with prepacked column and buffers

Item-Specific Features:

  • Agarose Resins and Kit are based on crosslinked 4% beaded agarose
  • UltraLink Resin is an acrylamide support (UltraLink Biosupport)

Includes:

Kit contains a pre-packed column of Monomeric Avidin Agarose and binding and elution buffers.

Documentation

Technical documents

Accessories and Spare Parts