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Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Brand:  Thermo Scientific™ 89879

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10311454

  • £49.41 / Pack of 50


EU Stock 4
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Description

Description

These microcentrifuge spin cups and spin columns are convenient tools for manipulating small volumes of affinity supports (5 to 500μL) for immunoprecipitation (IP) and pull-down purification assays. Five styles of polypropylene columns and matching collection tubes offer a variety of options to suit particular types of experimental needs. Add the affinity resin and sample to one of the columns and use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity-purify more protein in less time.

Highlights:

  • Efficient washing of samples means fewer washes are needed to remove contaminating proteins
  • Efficient elution of samples means better recovery of immunoprecipitation and assay products
  • Filter cups and columns eliminate contamination of eluted samples with affinity resin
  • Filter cups and columns eliminate the need to decant supernatant from pelleted debris or resin
  • Spin protocols drastically reduce the time required for small-scale affinity protocols
  • Low protein-binding polypropylene column construction minimizes nonspecific binding

Spin Cups-Paper Filter with Collection Tubes (Part No. 69700):

  • Column volume: 600μL
  • Resin volume: 20 to 300μL
  • Filter type: paper, 10μm pore size
  • Cap type: collection tube cap fits onto inserted spin cup

Spin Cups-Cellulose Acetate Filter with Collection Tubes (Part No. 69702):

  • Column volume: 800μL
  • Resin volume: 20 to 400μL
  • Filter type: cellulose acetate, 0.45μm pore size
  • Cap type: collection tube cap fits onto inserted spin cup
  • Spin ColumnsScrew Cap with Luer-Lok

Spin Columns-Screw Cap (Part No. 69705):

  • Column Volume: 900μL
  • Resin volume: 20 to 400μL
  • Filter type: polyethylene filter, 10μm pore size; small and large frit options for different sample sizes
  • Cap type: O-ring screw top caps; press-in bottom plugs
  • Luer-Lok Adapters enable use with syringe-based kits

Spin Columns-Snap Cap with Collection Tubes (Part No. 69725):

  • Column volume: 1000μL
  • Resin volume: 20 to 500μL
  • Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
  • Cap type: snap cap on column (no cap on collection tube); press-on bottom caps

Micro-Spin Columns (Part No. 89879):

  • Column volume: 400μL
  • Resin volume: 5 to 100μL
  • Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
  • Cap type: O-ring screw top caps; press-on bottom caps

Microcentrifuge Tubes (Part No. 69715):

  • 2mL capacity
  • Dolphin-style bottom with attached snap cap
  • Included and compatible with Part No. 69700

Microcentrifuge Tubes (Part No. 69720):

  • 2mL capacity
  • Short, conical bottom with attached snap cap
  • Included and compatible with Part No. 69702
Specifications

Specifications

Pierce Micro-Spin Columns
Polypropylene (400uL capacity); Polyethylene filter (30um pore size); Screw caps with O-rings; Press-in bottom plugs
50 columns
Microcentrifuge procedures with 5 to 100uL resin
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